Premium
ChIP ‐seq and transcriptome analysis of the OmpR regulon of S almonella enterica serovars T yphi and T yphimurium reveals accessory genes implicated in host colonization
Author(s) -
Perkins Timothy T.,
Davies Mark R.,
Klemm Elizabeth J.,
Rowley Gary,
Wileman Thomas,
James Keith,
Keane Thomas,
Maskell Duncan,
Hinton Jay C. D.,
Dougan Gordon,
Kingsley Robert A.
Publication year - 2013
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.12111
Subject(s) - biology , operon , regulon , salmonella enterica , chromatin immunoprecipitation , escherichia coli , genetics , gene , promoter , gene expression
Summary OmpR is a multifunctional DNA binding regulator with orthologues in many enteric bacteria that exhibits classical regulator activity as well as nucleoid‐associated protein‐like characteristics. In the enteric pathogen S almonella enterica , using chromatin immunoprecipitation of OmpR : FLAG and nucleotide sequencing, 43 putative OmpR binding sites were identified in S . enterica serovar T yphi, 22 of which were associated with OmpR ‐regulated genes. Mutation of a sequence motif ( TGTWACAW ) that was associated with the putative OmpR binding sites abrogated binding of OmpR :6×His to the tviA upstream region. A core set of 31 orthologous genes were found to exhibit OmpR ‐dependent expression in both S . T yphi and S . T yphimurium. S . T yphimurium‐encoded orthologues of two divergently transcribed OmpR ‐regulated operons ( SL 1068–71 and SL 1066–67) had a putative OmpR binding site in the inter‐operon region in S . T yphi, and were characterized using in vitro and in vivo assays. These operons are widely distributed within S . enterica but absent from the closely related E scherichia coli . SL 1066 and SL 1067 were required for growth on N ‐acetylmuramic acid as a sole carbon source. SL 1068–71 exhibited sequence similarity to sialic acid uptake systems and contributed to colonization of the ileum and caecum in the streptomycin‐pretreated mouse model of colitis.