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Hyphal heterogeneity in A spergillus oryzae is the result of dynamic closure of septa by W oronin bodies
Author(s) -
Bleichrodt RobertJan,
Veluw G. Jerre,
Recter Brand,
Maruyama Junichi,
Kitamoto Katsuhiko,
Wösten Han A. B.
Publication year - 2012
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/mmi.12077
Subject(s) - hypha , biology , cytoplasm , mycelium , green fluorescent protein , organelle , strain (injury) , microbiology and biotechnology , gene , botany , biochemistry , anatomy
Summary Hyphae of higher fungi are compartmentalized by septa. These septa contain a central pore that allows for inter‐compartmental and inter‐hyphal cytoplasmic streaming. The cytoplasm within the mycelium is therefore considered to be a continuous system. In this study, however, we demonstrate by laser dissection that 40% of the apical septa of exploring hyphae of A spergillus oryzae are closed. Closure of septa correlated with the presence of a peroxisome‐derived organelle, known as W oronin body, near the septal pore. The location of W oronin bodies in the hyphae was dynamic and, as a result, plugging of the septal pore was reversible. Septal plugging was abolished in a Δ Aohex1 strain that cannot form W oronin bodies. Notably, hyphal heterogeneity was also affected in the Δ Aohex1 strain. Wild‐type strains of A . oryzae showed heterogeneous distribution of GFP between neighbouring hyphae at the outer part of the colony when the reporter was expressed from the promoter of the glucoamylase gene glaA or the α‐glucuronidase gene aguA . In contrast, GFP fluorescence showed a normal distribution in the case of the Δ Aohex1 strain. Taken together, it is concluded that W oronin bodies maintain hyphal heterogeneity in a fungal mycelium by impeding cytoplasmic continuity.