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A Monoclonal Antibody that Binds Preferentially to Human Suppressor T Cells
Author(s) -
KATO Kotaro,
TERASIIIMA Masazumi,
OSAWA Toshiaki
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/mim.1982.26.7.599
Subject(s) - cytotoxic t cell , biology , concanavalin a , monoclonal antibody , microbiology and biotechnology , antigen , pokeweed mitogen , lymphoblast , t cell , peripheral blood mononuclear cell , interleukin 21 , antibody , t lymphocyte , cell culture , immunology , in vitro , immune system , biochemistry , genetics
A monoclonal antibody termed JM3‐3‐3A was produced by somatic cell hybridization. The reactivity was assessed by indirect immunofluorescence. JM3‐3‐3A was reactive with 41% human peripheral blood T cells and 48% non‐T cells. Among various human lymphoblastoid cell lines (MOLT 4F, JM, and TALL‐I), TALL‐I was found not to be reactive with JM‐13‐3‐3A. Human peripheral blood Tcells fractionated by JM3‐3‐3A‐coated dish panning were submitted to functional studies. JM3‐3‐3A positive T cells responded to mitogens, concanavalin A and phytohemagglutinin‐P much better than JM3‐3‐3A negative T cells in the presence or absence of adherent cells. JM3‐3‐3A positive T cells showed suppressor activity and JM3‐3‐3A negative T cells showed helper activity in pokeweed mitogen‐induced Ig production. More than fifty percent of the JM3‐3‐3A positive T cells were reactive with OKT8 which binds to suppressor/ cytotoxic T cells, whereas 18% of JM3‐3‐3A negative T cells were reactive with OKTIl. In addition, immunoprecipitation experiments identified a protein with an approximate molecular weight of 43,000 as a cell surface antigen for JM3‐3‐3A. Thus, the reactivity of JM3‐3‐3A showed a wide distribution but human peripheral blood T cells could be dissected functionally by this antibody.