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Expression of Target Antigen for Epstein‐Barr Virus‐Specific Cytotoxic T Cells on BJAB Cells Freshly Infected with EBV
Author(s) -
SUGAMURA Kazuo,
TANAKA Yuetsu,
HINUMA Yorio
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/mim.1982.26.7.575
Subject(s) - antigen , biology , cytotoxic t cell , virology , epstein–barr virus , virus , cell culture , major histocompatibility complex , microbiology and biotechnology , immunology , in vitro , biochemistry , genetics
The target antigen for Epstein‐Barr virus (EBV)‐specific cytotoxic T cells (Tc) was expressed on BJAB cells exposed to the B95‐8 strain of EBV for at least one hour. Ultraviolet‐light (UV)‐irradiated noninfectious B95‐8 virus also induced the target antigen on BJAB cells. Cold target competition tests suggested that the target antigen expressed on EBV‐infected BJAB cells was distinct from the lymphocyte‐detected membrane antigen (LYDMA) which was also recognized by the EBV‐specific Tc and expressed on autologous EBV‐transformed lymphoblastoid cell line (LCL) cells. Neither of these target antigens for EBV‐specific Tc was detected on the surface of EBV‐genome positive BJAB cells which had been kept in a long‐term culture after EBV‐infection. Thus, the virion antigen, especially the EBV‐membrane antigen (MA), is a possible candidate for the target antigen expressed on EBV‐infected BJAB cells. Lysis of EBV‐infected BJAB cells was inhibited by target cell treatment with anti‐ β 2 microglobulin (anti‐ β 2M) antibody and induction of the effector Tc was dependent on the donor individual. These resalts suggested the possibility that the Tc recognizing EBV‐infected BJAB cells are restricted by the major histocompatibility complex (MHC).