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Progressive ossification of the bone marrow vasculature with advancing age corresponds with reduced red blood cell count and percentage of circulating lymphocytes in male Fischer‐344 rats
Author(s) -
Guderian Sophie,
Lee Seungyong,
McLane Mary Ann,
Prisby Rhonda D.
Publication year - 2019
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12550
Subject(s) - bone marrow , ossification , medicine , hematocrit , cortical bone , anatomy , bone density , blood vessel , pathology , osteoporosis
Abstract Objective Assess the link between bone marrow blood vessel ossification, Tb. and cortical bone, and hematological parameters across the lifespan in rats. Methods Right femora and whole blood samples were taken from male Fischer‐344 rats at 1, 6, 12, 18 and 24 months. Femora were scanned by micro‐computed tomography (Micro CT ) to determine bone marrow blood vessel ossification (ie, ossified vessel volume [OsVV], ossified vessel thickness (OsV.Th), ossified vessel density (OsV density), and structural model index [ SMI ]). Bone microarchitecture (ie, bone volume [BV/TV], trabecular thickness, trabecular number, and trabecular separation), density and SMI , and cortical bone parameters (ie, cortical shell thickness, porosity, and density) were also determined by Micro CT . Complete blood counts with differentials were conducted. Results Ossified vessel volume increased throughout the lifespan, coinciding with reduced trabecular BV/TV and cortical shell thickness at 24 months. Many of the hematological parameters were unchanged (ie, white blood cells , lymphocyte number) or increased (monocyte number, percent monocyte, granulocyte number, percent granulocyte, hemoglobin, hematocrit, mean corpuscular hemoglobin concentration, red blood cell distribution width, platelet, mean platelet volume) with advancing age; however, red blood cells ( RBC ) and percent lymphocytes (LY%) were reduced at 24 months. In addition, OsV density was similar to trabecular bone density. Conclusions Declines in trabecular BV/TV, cortical shell thickness, RBC , and LY % with advanced age coincided with augmented ossification of bone marrow blood vessels.