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Capillary flow disturbances after experimental subarachnoid hemorrhage: A contributor to delayed cerebral ischemia?
Author(s) -
Anzabi Maryam,
Angleys Hugo,
Aamand Rasmus,
Ardalan Maryam,
Mouridsen Kim,
Rasmussen Peter Mondrup,
Sørensen Jens Christian Hedemann,
Plesnila Nikolaus,
Østergaard Leif,
Iversen Nina Kerting
Publication year - 2019
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12516
Subject(s) - vasospasm , capillary action , medicine , blood flow , perforation , ischemia , microcirculation , subarachnoid hemorrhage , perfusion , cerebral blood flow , anesthesia , blood capillary , cardiology , anatomy , circulatory system , materials science , punching , composite material , metallurgy
Background The high mortality and morbidity after SAH is partly due to DCI, which is traditionally ascribed to development of angiographic vasospasms. This relation has been challenged, and capillary flow disturbances are proposed as another mechanism contributing to brain damage after SAH . Objective To investigate capillary flow changes 4 days following experimental SAH . Methods SAH was induced by endovascular perforation of circle of Willis. We used TPM to evaluate blood flow characteristics. Cortical capillary diameters were investigated by both TPM and histology. Results We found elevated CTH and MTT of blood in SAH mice compared to sham animals. We observed capillaries with stagnant RBCs, and capillaries with increased RBC LD in the SAH group, suggesting severe blood maldistribution among cortical capillaries. Favoring that these capillary flow changes were primary to upstream vasoconstrictions, TPM showed no significant differences in arteriolar diameter between groups, while histological examination showed reduced capillary diameter in SAH group. Conclusion Our study shows profound subacute hypoperfusion and capillary flow disturbances in a mouse SAH model and suggests that these changes are the result of changes in capillary function, rather than upstream vasospasm.