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Differential arterial and venous endothelial redox responses to oxidative stress
Author(s) -
Shrestha Bandana,
Prasai Priya K.,
Kaskas Amir M.,
Khanna Ankur,
Letchuman Vijay,
Letchuman Sunjay,
Alexander Jonathan Steven,
Orr A. Wayne,
Woolard Matthew D.,
Pattillo Christopher B.
Publication year - 2018
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12486
Subject(s) - oxidative stress , redox , chemistry , medicine , biochemistry , organic chemistry
Objective Oxidative stress is a central event linked with endothelial dysfunction and inflammation in several vascular pathologies, marked by over‐production of ROS and concomitant decreases in antioxidants, for example GSH . Here, we distinguish endothelial oxidative stress regulation and associated functional disparities in the two main vascular conduits, (arteries and veins) following decreases in GSH . Methods MAEC s and VCEC s were used as models of arterial and venular endothelium, respectively, and BSO (0‐100 μmol/L) was used to indirectly increase cellular oxidative stress. Inflammatory responses were measured using immune cell attachment and immunoblotting for endothelial cell adhesion molecule (ICAM‐1, VCAM‐1) expression, altered cell proliferation, and wound healing. Results MAEC s and VCEC s exhibited differential responses to oxidative stress produced by GSH depletion with VCEC s exhibiting greater sensitivity to oxidative stress. Compared to MAEC s, VCEC s showed a significantly increased inflammatory profile and a decreased proliferative phenotype in response to decreases in GSH levels. Conclusions Arterial and venous endothelial cells exhibit differential responses to oxidant stress, and decreases in GSH : GSSG are more exacerbated in venous endothelial cells. Specific pathogenesis in these vascular conduits, with respect to oxidant stress handling, warrants further study, especially considering surgical interventions such as Coronary artery bypass grafting that use both interchangeably.