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Hypoxia‐enhanced adhesion of red blood cells in microscale flow
Author(s) -
Kim Myeongseop,
Alapan Yunus,
Adhikari Anima,
Little Jane A.,
Gurkan Umut A.
Publication year - 2017
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12374
Subject(s) - microscale chemistry , adhesion , hypoxia (environmental) , microcirculation , population , oxygen tension , blood flow , biophysics , red blood cell , cell adhesion , chemistry , microfluidics , microbiology and biotechnology , biomedical engineering , pathology , immunology , medicine , biology , nanotechnology , materials science , oxygen , biochemistry , mathematics education , mathematics , environmental health , organic chemistry
Objectives The advancement of microfluidic technology has facilitated the simulation of physiological conditions of the microcirculation, such as oxygen tension, fluid flow, and shear stress in these devices. Here, we present a micro‐gas exchanger integrated with microfluidics to study RBC adhesion under hypoxic flow conditions mimicking postcapillary venules. Methods We simulated a range of physiological conditions and explored RBC adhesion to endothelial or subendothelial components ( FN or LN ). Blood samples were injected into microchannels at normoxic or hypoxic physiological flow conditions. Quantitative evaluation of RBC adhesion was performed on 35 subjects with homozygous SCD . Results Significant heterogeneity in RBC adherence response to hypoxia was seen among SCD patients. RBC s from a HEA population showed a significantly greater increase in adhesion compared to RBC s from a HNA population, for both FN and LN . Conclusions The approach presented here enabled the control of oxygen tension in blood during microscale flow and the quantification of RBC adhesion in a cost‐efficient and patient‐specific manner. We identified a unique patient population in which RBC s showed enhanced adhesion in hypoxia in vitro. Clinical correlates suggest a more severe clinical phenotype in this subgroup.