Altered distribution of adrenergic constrictor responses contributes to skeletal muscle perfusion abnormalities in metabolic syndrome

Purpose Although studies suggest elevated adrenergic activity paralleling metabolic syndrome in OZR s, the moderate hypertension and modest impact on organ perfusion question the multi‐scale validity of these data. Methods To understand how adrenergic function contributes to vascular reactivity in OZR , we utilized a multi‐scale approach to investigate pressure responses, skeletal muscle blood flow, and vascular reactivity following adrenergic challenge. Results For OZR , adrenergic challenge resulted in increased pressor responses vs LZR s, mediated via α 1 receptors, with minimal contribution by either ROS or NO bioavailability. In situ gastrocnemius muscle of OZR exhibited blunted functional hyperemia, partially restored with α 1 inhibition, although improved muscle performance and VO 2 required combined treatment with TEMPOL . Within OZR in situ cremaster muscle, proximal arterioles exhibited a more heterogeneous constriction to adrenergic challenge, biased toward hyperresponsiveness, vs LZR . This increasingly heterogeneous pattern was mirrored in ex vivo arterioles, mediated via α 1 receptors, with roles for ROS and NO bioavailability evident in hyperresponsive vessels only. Conclusions These results support the central role of the α 1 adrenoreceptor for augmented pressor responses and elevations in vascular resistance, but identify an increased heterogeneity of constrictor reactivity in OZR that is presently of unclear purpose.