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Arteriogenesis in murine adipose tissue is contingent on CD68 + /CD206 + macrophages
Author(s) -
Seaman Scott A.,
Cao Yiqi,
Campbell Chris A.,
Peirce Shayn M.
Publication year - 2017
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12341
Subject(s) - arteriogenesis , adipose tissue , perfusion , macrophage , medicine , transplantation , cd68 , surgery , pathology , chemistry , cancer research , angiogenesis , in vitro , immunohistochemistry , biochemistry
Objective The surgical transfer of skin, fat, and/or muscle from a donor site to a recipient site within the same patient is a widely performed procedure in reconstructive surgeries. A surgical pretreatment strategy that is intended to increase perfusion in the flap, termed “flap delay,” is a commonly employed technique by plastic surgeons prior to flap transplantation. Here, we explored whether CD 68 + / CD 206 + macrophages are required for arteriogenesis within the flap by performing gain‐of‐function and loss‐of‐function studies in a previously published flap delay murine model. Methods and Results Local injection of M2‐polarized macrophages into the flap resulted in an increase in collateral vessel diameter. Application of a thin biomaterial film loaded with a pharmacological agent ( FTY 720), which has been previously shown to recruit CD 68 + / CD 206 + macrophages to remodeling tissue, increased CD 68 + / CD 206 + cell recruitment and collateral vessel enlargement. Conversely, when local macrophage populations were depleted within the inguinal fat pad via clodronate liposome delivery, we observed fewer CD 68 + cells accompanied by diminished collateral vessel enlargement. Conclusions Our study underscores the importance of macrophages during microvascular adaptations that are induced by flap delay. These studies suggest a mechanism for a translatable therapeutic target that may be used to enhance the clinical flap delay procedure.