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Kudiezi Injection ® Alleviates Blood–Brain Barrier Disruption After Ischemia‐Reperfusion in Rats
Author(s) -
Chen FuQin,
Li Quan,
Pan ChunShui,
Liu YuYing,
Yan Li,
Sun Kai,
Mao XiaoWei,
Mu HongNa,
Wang MingXia,
Wang ChuanShe,
Fan JingYu,
Cui YuanChen,
Zhang YunPei,
Yang JiaYi,
Bai Wen,
Han JingYan
Publication year - 2016
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12288
Subject(s) - extravasation , evans blue , proto oncogene tyrosine protein kinase src , blood–brain barrier , caveolin 1 , albumin , ischemia , phosphorylation , chemistry , pharmacology , cerebral infarction , medicine , anesthesia , biochemistry , pathology , central nervous system
Objective This study was designed to examine the effect of KDZ , on the BBB disruption in rat underwent MCAO and reperfusion. Methods Male Sprague–Dawley rats (260–280 g) were subjected to 60 minutes MCAO followed by reperfusion. KDZ (4 mL/kg) was administrated before ischemia. The Evans blue extravasation, albumin leakage, brain water content, TJ proteins, caveolin‐1, p‐caveolin‐1, Src, and p‐Src were evaluated. Neurological scores, cerebral infarction, and CBF were assessed. The binding affinity of KDZ to Src was examined. Results I/R evoked a range of insults including Evans blue extravasation, albumin leakage, brain water content increase, CBF decrease, cerebral infarction, and neurological deficits, all of which were attenuated by KDZ . Meanwhile, KDZ inhibited TJ proteins down‐expression, expression of caveolin‐1, phosphorylation of caveolin‐1 and Src after I/R. In addition, SPR revealed binding of KDZ to Src with high affinity. Conclusions KDZ protects BBB from disruption and improves cerebral outcomes following I/R via preventing the degradation of TJ proteins, caveolin‐1 expression, and inhibiting p‐caveolin‐1 and p‐Src, which were most likely attributable to the ability of its main ingredients to bind to Src and inhibit its phosphorylation.