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Exercise and Repeated Testing Improves Accuracy of Laser Doppler Assessment of Microvascular Function Following Shortened (1‐minute) Blood Flow Occlusion
Author(s) -
Tran Brian D.,
Chiu Abraham,
Tran Charlene,
Rogacion Danica Rose,
Tfaye Nicole,
Ganesan Goutham,
Galassetti Pietro R.
Publication year - 2016
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12277
Subject(s) - occlusion , medicine , laser doppler velocimetry , blood flow , microcirculation , reactive hyperemia , perfusion , cardiology , nuclear medicine
Objectives To determine whether stability/accuracy of post‐occlusive LDF following shortened, one‐minute blood flow occlusion, increases in the post‐exercise state or by averaging multiple measurements. Methods Six healthy adults (3F) underwent LDF eight times at rest and following exercise, assessing post‐occlusive (one‐minute occlusion) reactive hyperemia in the cutaneous microcirculation of the forefinger. Measured variables included: pre‐ and post‐occlusion steady‐state perfusion (Plat1, Plat2), maximum post‐occlusive perfusion (Max), PkT, and the ratio Max/Plat1. Results Stability/accuracy of all variables improved performing measurements after exercise ( p < 0.05 Plat 1, Plat 2, Max and Max/Plat1). PkT and Max/Plat 1 displayed the greatest accuracy at rest (26.6 ± 5.1% and 26.6 ± 4.4% average difference, %Diff, of single measurements from individual “true” means, respectively); for these variables, %Diff improved to 19.5 ± 5.3 and 17.6 ± 2.1, respectively, following exercise. Overall, averaging multiple measurements performed at rest also improved stability/accuracy in all variables. This improvement was comparable to that obtained with a single measurement following exercise. Conclusions A standardized exercise stimulus prior to testing significantly improves stability/accuracy of LDF following shortened, one‐minute blood flow occlusion. Our results suggest the possibilities of broader applications of exercise to optimize measurements from a variety of skin perfusion methodologies.