Premium
Adiponectin Receptor Agonist, AdipoRon, Causes Vasorelaxation Predominantly Via a Direct Smooth Muscle Action
Author(s) -
Hong Kwangseok,
Lee Sewon,
Li Rong,
Yang Yan,
Tanner Miles A.,
Wu Jianbo,
Hill Michael A.
Publication year - 2016
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12266
Subject(s) - adiponectin receptor 1 , vasodilation , endocrinology , medicine , electrical impedance myography , adiponectin , chemistry , cerebral arteries , vasoconstriction , vascular smooth muscle , mesenteric arteries , biology , artery , insulin , insulin resistance , smooth muscle
Objective AdipoRon, an adiponectin receptor agonist, was recently proposed for treating insulin resistance and hyperglycemia. As adiponectin is vasoprotective via NO ‐mediated signaling, it was hypothesized that adipoRon similarly exerts potentially beneficial vasodilator effects. We therefore examined if adipoRon induces vasorelaxation and via what contributing mechanisms. Methods Vascular function was assessed in skeletal muscle arteries from rats and cerebral/coronary arteries from mice using pressure and wire myography. Results Using qPCR , mRNA for adiponectin receptors was demonstrated in skeletal muscle, cerebral and coronary arteries. AdipoRon‐caused vasorelaxation was not abolished by compound C (10 μ M; AMPK inhibitor). Inhibition of endothelium‐dependent relaxation with combinations of l ‐ NAME /indomethacin/apamin/ TRAM ‐34 only slightly reduced adipoRon‐mediated vasorelaxation in cerebral and coronary arteries. EC ‐denuded cremaster arteries showed similar relaxant responses to adipoRon as in intact vessels, suggesting adipoRon directly impacts VSMC s. K + currents measured in VSMC s isolated from mouse basilar and LAD arteries were not altered by adiopRon. In cremaster arteries, adipoRon induced vasorelaxation without a marked decrease in VSMC [Ca 2+ ] i . Adiponectin, itself, caused vasodilation in intact cremaster arteries while failing to cause significant dilation in EC ‐denuded arteries, consistent with endothelium dependency of adiponectin. Conclusions AdipoRon exerts vasodilation by mechanisms distinct to adiponectin. The dominant mechanism for adipoRon‐induced vasorelaxation occurs independently of endothelium‐dependent relaxing factors, AMPK activation, K + efflux‐mediated hyperpolarization and reductions in cytosolic [Ca 2+ ] i .