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High Concentrations of Uric Acid Inhibit Endothelial Cell Migration via miR‐663 Which Regulates Phosphatase and Tensin Homolog by Targeting Transforming Growth Factor‐ β 1
Author(s) -
Hong Quan,
Yu Shandong,
Geng Xiaodong,
Duan Liping,
Zheng Wei,
Fan Men,
Chen Xiangmei,
Wu Di
Publication year - 2015
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12200
Subject(s) - pten , tensin , cancer research , cell migration , transfection , microrna , endothelial stem cell , transforming growth factor , small interfering rna , cell growth , chemistry , cell , biology , microbiology and biotechnology , pi3k/akt/mtor pathway , cell culture , signal transduction , gene , biochemistry , genetics , in vitro
Background Whether microRNAs participate in endothelial dysfunction HUA remains unknown. A previous study indicated that miR‐663 was the most significantly differentially expressed endothelial microRNA under HUA conditions. Some studies have demonstrated that the miR‐663 target gene and TGF ‐ β 1, promoted endothelial cell migration by inhibiting PTEN deleted on chromosome 10. Therefore, we hypothesized that HUA inhibits endothelial migration via miR‐663, which regulates PTEN by targeting TGF ‐ β 1. Methods PCR analysis was performed to determine miR‐663 expression levels. A luciferase assay was performed to validate whether miR‐663 targets TGF ‐ β 1 directly. Western blot analysis was performed to determine TGF ‐ β 1 and PTEN expression levels. An miR‐663 inhibitor and TGF ‐ β 1‐ and PTEN ‐specific siRNAs were transfected into EA.hy926 cells to inhibit miR‐663, TGF ‐ β 1, and PTEN expression, respectively. A wound healing assay was performed to determine the migratory ability of EA.hy926 cells. Results miR‐663 had higher expression levels in HUA ‐stimulated endothelial cells and in the sera of hyperuricemic patients and animals. TGF ‐ β 1 was targeted directly by miR‐663. Endothelial miR‐663 was up‐regulated under HUA conditions, and HUA inhibited endothelial cell migration via miR‐663, which targeted TGF‐ β 1. Thus, TGF‐ β 1 regulated cell migration in a PTEN ‐dependent manner. Conclusion HUA inhibits endothelial cell migration via miR‐663, which regulates PTEN by targeting TGF ‐ β 1.