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Pretreatment with Andrographolide Pills ® Attenuates Lipopolysaccharide‐Induced Pulmonary Microcirculatory Disturbance and Acute Lung Injury in Rats
Author(s) -
Yang Ning,
Liu YuYing,
Pan ChunShui,
Sun Kai,
Wei XiaoHong,
Mao XiaoWei,
Lin Fang,
Li XueJun,
Fan JingYu,
Han JingYan
Publication year - 2014
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12152
Subject(s) - lipopolysaccharide , medicine , pharmacology , microvessel , pulmonary edema , oxidative stress , lung , edema , andrographolide , vascular permeability , nadph oxidase , immunology , immunohistochemistry
Objective The purpose of this study was to explore the protective effect of AP on LPS ‐induced PMD and ALI . Methods Male SD rats were continuously infused with LPS (5 mg/kg/h) for one hour to induce PMD and ALI . AP was administrated orally one hour before LPS exposure. Arterial blood pressure and HR were monitored. Blood gas analysis, histological observation, cytokines in plasma, leukocyte recruitment, pulmonary oxidative stress, microvessel permeability, edema, and related proteins were evaluated six hours after LPS challenge. Results Rats receiving LPS exhibited significant alterations, including hypotension, tachycardia, increase in cytokines, neutrophil adhesion and infiltration, oxidative stress, and microvessel hyperpermeability, resulting in pulmonary injury and dysfunction. AP (0.18 g/kg or 1.8 g/kg) improved rat survival rate, and significantly attenuated all aforementioned insults, and inhibited LPS ‐induced increase in adhesion molecules, up‐regulation of Cav‐1 and Src kinase and NADPH oxidase subunits (p47 phox and p67 phox ) membrane translocation in lung tissue, and preserved JAM‐1 and claudin‐5. Conclusions The results demonstrated the protective effect of AP on LPS ‐induced PMD and ALI , suggesting the potential of AP as a prophylactic strategy for LPS ‐induced ALI .