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Impact of Increased Intramuscular Perfusion Heterogeneity on Skeletal Muscle Microvascular Hematocrit in the Metabolic Syndrome
Author(s) -
Butcher Joshua T.,
Stanley Shyla C.,
Brooks Steven D.,
Chantler Paul D.,
Wu Fan,
Frisbee Jefferson C.
Publication year - 2014
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12146
Subject(s) - skeletal muscle , perfusion , medicine , microcirculation , phentolamine , endocrinology , chemistry , cardiology , receptor
Objective To determine H MV and PS in skeletal muscle of OZR and evaluate the impact of increased microvascular perfusion heterogeneity on mass transport/exchange. Methods The in situ gastrocnemius muscle from OZR and LZR was examined under control conditions and following pretreatment with TEMPOL (antioxidant)/ SQ ‐29548 ( PGH 2 /TxA 2 receptor antagonist), phentolamine (adrenergic antagonist), or all agents combined. A spike input of a labeled blood tracer cocktail was injected into the perfusing artery. Tracer washout was analyzed using models for H MV and PS . H T was determined in in situ cremaster muscle of OZR and LZR using videomicroscopy. Results H MV was decreased in OZR versus LZR . While TEMPOL / SQ ‐29548 or phentolamine had minor effects, treatment with all three agents improved H MV in OZR . H T was not different between strains, although variability was increased in OZR , and normalized following treatment with all three agents. PS was reduced in OZR and was not impacted by intervention. Conclusions Increased microvascular perfusion heterogeneity in OZR reduces H MV in muscle vascular networks and increases its variability, potentially contributing to premature muscle fatigue. While targeted interventions can ameliorate this, the reduced microvascular surface area is not acutely reversible.