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Involvement of H1 and H2 Receptors and Soluble Guanylate Cyclase in Histamine‐Induced Relaxation of Rat Mesenteric Collecting Lymphatics
Author(s) -
Kurtz Kristine H.,
Moor Andrea N.,
SouzaSmith Flavia M.,
Breslin Jerome W.
Publication year - 2014
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12138
Subject(s) - histamine , histamine h2 receptor , cimetidine , histamine h1 receptor , histamine receptor , chemistry , endocrinology , histamine h4 receptor , medicine , histamine n methyltransferase , histamine h3 receptor , receptor , pharmacology , antagonist , biology
Objective This study investigated the roles of the H1 and H2 histamine receptors, NO synthase, and s GC cyclase in histamine‐induced modulation of rat mesenteric collecting lymphatic pumping. Methods Isolated rat mesenteric collecting lymphatics were treated with 1‐ to 100‐ μ M histamine. Histamine receptors were blocked with either the H1 antagonist mepyramine or the H2 antagonist cimetidine. The role of NO /s GC signaling was tested using the arginine analog l ‐ NAME , the s GC inhibitor ODQ , and SNP as a positive control. Results Histamine applied at 100 μ M decreased tone and CF of isolated rat mesenteric collecting lymphatics. Pharmacologic blockade of either H1 or H2 histamine receptors significantly inhibited the response to histamine. Pretreatment with ODQ , but not l ‐ NAME , completely inhibited the histamine‐induced decrease in tone. ODQ pretreatment also significantly inhibited SNP ‐induced lymphatic relaxation. Conclusions H1 and H2 histamine receptors are both involved in histamine‐induced relaxation of rat mesenteric collecting lymphatics. NO synthesis does not appear to contribute to the histamine‐induced response. However, s GC is critical for the histamine‐induced decrease in tone and contributes to the drop in CF .