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Improvement in Middle Cerebral Artery Structure and Endothelial Function in Stroke‐Prone Spontaneously Hypertensive Rats after Macrophage Depletion
Author(s) -
Pires Paulo W.,
Girgla Saavia S.,
McClain Jonathon L.,
Kaminski Norbert E.,
Rooijen Nico,
Dorrance Anne M.
Publication year - 2013
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12064
Subject(s) - electrical impedance myography , medicine , middle cerebral artery , lumen (anatomy) , endothelium , inflammation , vasodilation , cerebral arteries , stroke (engine) , cerebral circulation , blood pressure , macrophage , perfusion , endocrinology , ischemia , in vitro , chemistry , biochemistry , mechanical engineering , engineering
Background Inflammation is involved in the pathogenesis of hypertension. Hypertensive animals have an increased number of perivascular macrophages in cerebral arteries. Macrophages might be involved in remodeling of the cerebral vasculature. We hypothesized that peripheral macrophage depletion would improve MCA structure and function in hypertensive rats. Methods For macrophage depletion, six‐week‐old stroke‐prone spontaneously hypertensive rats ( SHRSP ) were treated with CLOD , 10 mL/kg every three or four days, i.p., or vehicle ( PBS lipo). MCA structure and function were analyzed by pressure and wire myography. Results Blood pressure was not affected by CLOD . The number of perivascular CD 163‐positive cells per microscopic field was reduced in the brain of SHRSP + CLOD . CLOD treatment caused an improvement in endothelium‐dependent dilation after intralumenal perfusion of ADP and incubation with Ach. Inhibition of NO production blunted the Ach response, and endothelium‐independent dilation was not altered. At an intralumenal pressure of 80 mmHg, MCA from SHRSP + CLOD showed increased lumen diameter, decreased wall thickness, and wall‐to‐lumen ratio. Cross‐sectional area of pial arterioles from SHRSP + CLOD was higher than PBS lipo. Conclusions These results suggest that macrophage depletion attenuates MCA remodeling and improves MCA endothelial function in SHRSP .