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Emodin Improves Lipopolysaccharide‐Induced Microcirculatory Disturbance in Rat Mesentery
Author(s) -
Li Ang,
Dong Lei,
Duan MeiLi,
Sun Kai,
Liu YuYing,
Wang MingXia,
Deng JingNa,
Fan JingYu,
Wang BaoEn,
Han JingYan
Publication year - 2013
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/micc.12061
Subject(s) - emodin , pharmacology , lipopolysaccharide , microcirculation , chemistry , inflammation , medicine , myeloperoxidase , sepsis , pathology , immunology , biochemistry
Objective Sepsis is a systemic inflammatory response syndrome. Emodin is a major ingredient of Rheum Palmatum , a Chinese herb that is widely used in China for treatment of endotoxemia‐related diseases. This study intended to examine the effect of Emodin on LPS ‐induced rat mesenteric microcirculatory disturbance and the underlying mechanisms. Methods The male Wistar rats received LPS (5 mg/kg/hr) for 90 min, with or without administration of Emodin (10 mg/kg/hr) by enema 30 min before (pre‐treatment) or after (post‐treatment) LPS infusion, and the dynamics of mesenteric microcirculation were determined by inverted intravital microscopy. Expression of adhesion molecules and TLR 4, NF ‐κB p65, ICAM ‐1, MPO , and AP ‐1 in mesentery tissue was evaluated by flow cytometry and Western‐blot, respectively. Results Pre or post‐treatment with Emodin significantly ameliorated LPS ‐induced leukocyte emigration, reactive oxygen species production and albumin leakage, and the expression of TLR 4, NF ‐κB p65, ICAM ‐1, MPO and AP ‐1 in mesentery. Conclusions These results demonstrate the beneficial role of Emodin in attenuating the LPS ‐induced microcirculatory disturbance, and support the use of Emodin for patients with endotoxemia.