Premium
Environmental DNA metabarcoding of cow dung reveals taxonomic and functional diversity of invertebrate assemblages
Author(s) -
Sigsgaard Eva Egelyng,
Olsen Kent,
Hansen Morten D. D.,
Hansen Oskar Liset Pryds,
Høye Toke Thomas,
Svenning JensChristian,
Thomsen Philip Francis
Publication year - 2021
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/mec.15734
Subject(s) - biology , invertebrate , environmental dna , ecology , herbivore , species richness , habitat , biodiversity , abundance (ecology)
Insects and other terrestrial invertebrates are declining in species richness and abundance. This includes the invertebrates associated with herbivore dung, which have been negatively affected by grazing abandonment and the progressive loss of large herbivores since the Late Pleistocene. Importantly, traditional monitoring of these invertebrates is time‐consuming and requires considerable taxonomic expertise, which is becoming increasingly scarce. In this study, we investigated the potential of environmental DNA (eDNA) metabarcoding of cow dung samples for biomonitoring of dung‐associated invertebrates. From eight cowpats we recovered eDNA from 12 orders, 29 families, and at least 54 species of invertebrates (mostly insects), representing several functional groups. Furthermore, species compositions differed between the three sampled habitats of dry grassland, meadow, and forest. These differences were in accordance with the species’ ecology; for instance, several species known to be associated with humid conditions or lower temperatures were found only in the forest habitat. We discuss potential caveats of the method, as well as directions for future study and perspectives for implementation in research and monitoring.