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Rhizosphere‐associated Pseudomonas induce systemic resistance to herbivores at the cost of susceptibility to bacterial pathogens
Author(s) -
Haney Cara H.,
Wiesmann Christina L.,
Shapiro Lori R.,
Melnyk Ryan A.,
O'Sullivan Lucy R.,
Khorasani Sophie,
Xiao Li,
Han Jiatong,
Bush Jenifer,
Carrillo Juli,
Pierce Naomi E.,
Ausubel Frederick M.
Publication year - 2018
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/mec.14400
Subject(s) - biology , rhizosphere , pseudomonas syringae , pseudomonas , trichoplusia , jasmonic acid , microbiology and biotechnology , salicylic acid , arabidopsis , pseudomonadaceae , pathogen , cabbage looper , systemic acquired resistance , pseudomonadales , botany , bacteria , genetics , gene , mutant , pest analysis , noctuidae
Plant‐associated soil microbes are important mediators of plant defence responses to diverse above‐ground pathogen and insect challengers. For example, closely related strains of beneficial rhizosphere Pseudomonas spp. can induce systemic resistance ( ISR ), systemic susceptibility ( ISS ) or neither against the bacterial foliar pathogen Pseudomonas syringae pv. tomato DC 3000 ( Pto DC 3000). Using a model system composed of root‐associated Pseudomonas spp. strains, the foliar pathogen Pto DC 3000 and the herbivore Trichoplusia ni (cabbage looper), we found that rhizosphere‐associated Pseudomonas spp. that induce either ISS and ISR against Pto DC 3000 all increased resistance to herbivory by T. ni . We found that resistance to T. ni and resistance to Pto DC 3000 are quantitative metrics of the jasmonic acid ( JA )/salicylic acid ( SA ) trade‐off and distinct strains of rhizosphere‐associated Pseudomonas spp. have distinct effects on the JA / SA trade‐off. Using genetic analysis and transcriptional profiling, we provide evidence that treatment of Arabidopsis with Pseudomonas sp. CH 267, which induces ISS against bacterial pathogens, tips the JA / SA trade‐off towards JA ‐dependent defences against herbivores at the cost of a subset of SA ‐mediated defences against bacterial pathogens. In contrast, treatment of Arabidopsis with the ISR strain Pseudomonas sp. WCS 417 disrupts JA / SA antagonism and simultaneously primes plants for both JA ‐ and SA ‐mediated defences. Our findings show that ISS against the bacterial foliar pathogens triggered by Pseudomonas sp. CH 267 , which is a seemingly deleterious phenotype, may in fact be an adaptive consequence of increased resistance to herbivory. Our work shows that pleiotropic effects of microbiome modulation of plant defences are important to consider when using microbes to modify plant traits in agriculture.