Premium
Mosquitoes rely on their gut microbiota for development
Author(s) -
Coon Kerri L.,
Vogel Kevin J.,
Brown Mark R.,
Strand Michael R.
Publication year - 2014
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/mec.12771
Subject(s) - biology , axenic , aedes aegypti , microbiome , anopheles gambiae , larva , zoology , pyrosequencing , bacteria , ecology , malaria , bioinformatics , biochemistry , genetics , gene , immunology
Field studies indicate adult mosquitoes ( C ulicidae) host low diversity communities of bacteria that vary greatly among individuals and species. In contrast, it remains unclear how adult mosquitoes acquire their microbiome, what influences community structure, and whether the microbiome is important for survival. Here, we used pyrosequencing of 16S r RNA to characterize the bacterial communities of three mosquito species reared under identical conditions. Two of these species, A edes aegypti and A nopheles gambiae , are anautogenous and must blood‐feed to produce eggs, while one, G eorgecraigius atropalpus , is autogenous and produces eggs without blood feeding. Each mosquito species contained a low diversity community comprised primarily of aerobic bacteria acquired from the aquatic habitat in which larvae developed. Our results suggested that the communities in A e. aegypti and A n. gambiae larvae share more similarities with one another than with G . atropalpus . Studies with A e. aegypti also strongly suggested that adults transstadially acquired several members of the larval bacterial community, but only four genera of bacteria present in blood fed females were detected on eggs. Functional assays showed that axenic larvae of each species failed to develop beyond the first instar. Experiments with A e. aegypti indicated several members of the microbial community and E scherichia coli successfully colonized axenic larvae and rescued development. Overall, our results provide new insights about the acquisition and structure of bacterial communities in mosquitoes. They also indicate that three mosquito species spanning the breadth of the C ulicidae depend on their gut microbiome for development.