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River islands, refugia and genetic structuring in the endemic brown frog R ana kukunoris ( A nura, R anidae) of the Q inghai‐ T ibetan P lateau
Author(s) -
Zhou Weiwei,
Yan Fang,
Fu Jinzhong,
Wu Shifang,
Murphy Robert W.,
Che Jing,
Zhang Yaping
Publication year - 2013
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/mec.12087
Subject(s) - biology , refugium (fishkeeping) , last glacial maximum , lineage (genetic) , phylogeography , genetic structure , ecology , genetic diversity , pleistocene , glacial period , genetic variation , habitat , zoology , phylogenetics , gene , population , genetics , paleontology , demography , sociology
Frequently, P leistocene climatic cycling has been found to be the diver of genetic structuring in populations, even in areas that did not have continental ice sheets, such as on the Q inghai‐ T ibetan P lateau ( QTP ). Typically, species distributed on the plateau have been hypothesized to re‐treat to south‐eastern refugia, especially during the L ast G lacial M aximum ( LGM ). We evaluated sequence variation in the mitochondrial DNA gene Cytb and the nuclear DNA gene RAG ‐1 in R ana kukunoris , a species endemic to the QTP . Two major lineages, N and S, were identified, and lineage N was further subdivided into N 1 and N 2. The geographical distribution and genealogical divergences supported the hypothesis of multiple refugia. However, major lineages and sublineages diverged prior to the LGM . Demographical expansion was detected only in lineage S and sublineage N 2. Sublineage N 1 might have survived several glacial cycles in situ and did not expand after the LGM because of the absence of suitable habitat; it survived in river islands. Genetic analysis and environment modelling suggested that the north‐eastern edge of QTP contained a major refugium for R . kukunoris . From here, lineage S dispersed southwards after the LGM . Two microrefugia in northern Q ilian M ountains greatly contributed to current level of intraspecific genetic diversity. These results were found to have important implications for the habitat conservation in N orthwest C hina.