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Can light intensity cause shifts in natural product and bacterial profiles of the sponge A plysina aerophoba ?
Author(s) -
SacristánSoriano Oriol,
Banaigs Bernard,
Becerro Mikel A.
Publication year - 2016
Publication title -
marine ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 58
eISSN - 1439-0485
pISSN - 0173-9565
DOI - 10.1111/maec.12252
Subject(s) - sponge , phylotype , holobiont , temperature gradient gel electrophoresis , biology , bacteria , chemical ecology , amplicon sequencing , abundance (ecology) , 16s ribosomal rna , host (biology) , ecology , chemistry , microbiology and biotechnology , symbiosis , botany , genetics
The potential role of microbial consortia on sponge chemistry is well known. However, how environmental factors affect microbial and chemical profiles and how these shifts affect the sponge holobiont are far from being understood. This study experimentally investigated the effect of light on both the concentration of secondary metabolites and the bacterial assemblages of the sponge Aplysina aerophoba . We quantified major brominated alkaloids ( BA s) using a high‐performance liquid chromatography system coupled with a UV ‐detector and analysed sponge‐associated bacteria using denaturing gradient gel electrophoresis of 16S r RNA gene amplicons. We identified distinct chemical and bacterial profiles between the ectosome and the choanosome of A. aerophoba . The abundance of most secondary metabolites increased regardless of the illumination regime. We found that the probability of occurrence of three microbial phylotypes (operational taxonomic units 84, 86 and 87) was strongly associated with increasing concentrations of three brominated compounds (aerophobin‐1, aplysinamisin‐1 and isofistularin‐3). Although the role of these bacteria remains uninvestigated, these associations between natural products and specific microbial phylotypes outline further hypotheses that will improve our understanding of the organization and functioning of these complex host–symbiont interactions.