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Genotyping of Rhodopirellula baltica organisms using multispacer sequence typing
Author(s) -
Cayrou Caroline,
Terra Anthony,
Drancourt Michel
Publication year - 2013
Publication title -
marine ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 58
eISSN - 1439-0485
pISSN - 0173-9565
DOI - 10.1111/maec.12015
Subject(s) - baltica , genotyping , biology , typing , genetics , strain (injury) , sequence (biology) , evolutionary biology , gene , genotype , paleontology , anatomy , ordovician
Rhodopirellula baltica is a ubiquitous P lanctomycetes organism isolated from marine environments worldwide. Multilocus sequence analysis ( MLSA ) has been the only method used to assess the diversity of R. baltica organisms. Here, we developed multispacer sequence typing ( MST ) genotyping and applied it to four R. baltica isolates from the F rench M editerranean S ea and R. baltica DSM 10527 T type strain. While MLSA yielded interpretable sequences for two of nine genes in three R. baltica isolates, MST based on sequencing two intergenic spacers yielded an unambiguous, strain‐specific profile for each one of the four R. baltica isolates under investigation. We propose that MST could be used as an alternative R. baltica genotyping method to assess the diversity of these P lanctomycetes organisms.