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The pretreatment of rats with nebivolol ameliorates bladder contractile dysfunction caused by ischemia‐reperfusion injury
Author(s) -
AltunkaynakCamca Hande O.,
Yazihan Nuray
Publication year - 2021
Publication title -
luts: lower urinary tract symptoms
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.451
H-Index - 15
eISSN - 1757-5672
pISSN - 1757-5664
DOI - 10.1111/luts.12338
Subject(s) - nebivolol , carbachol , oxidative stress , concomitant , ischemia , urinary bladder , medicine , chemistry , reperfusion injury , anesthesia , endocrinology , pharmacology , stimulation , blood pressure
Objective The present study aimed to investigate the protective effect of nebivolol in the bladder isolated from rats exposed to ischemia‐reperfusion (IR) injury. Methods Sprague‐Dawley rats were divided into control, IR, and nebivolol+IR groups. In the nebivolol+IR group, nebivolol was administered (0.4 mg/kg, subcutaneous) in rats prior to IR insult. At the end of the experimental protocol, the urinary bladder was rapidly isolated and bladder strips were mounted in an organ bath. After the equilibration period, potassium chloride (KCl, 20‐100 mM) or carbachol (0.01‐10 μM) was cumulatively added to the organ bath to generate cumulative concentration‐response curves (CCRCs). Oxidative stress and interleukin 6 (IL‐6) levels were also evaluated in the bladder tissue. Results The CCRCs of KCl and carbachol were significantly reduced in the IR group compared to those of the control, and this inhibition was reversed by the pretreatment of rats with nebivolol ( P < .05). The IR group's total antioxidant status was significantly lower with a concomitant increase in IL‐6 levels than that of the control and nebivolol+IR groups ( P < .05). Conclusions The present study indicates that pretreatment of rats with nebivolol (0.4 mg/kg) could improve bladder contractile dysfunction caused by IR injury through suppression of increased oxidative stress and IL‐6 levels.