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Tadalafil, a phosphodiesterase type 5 inhibitor, restores urethra and detrusor function in the initial phase of diabetes in rats
Author(s) -
Gotoh Daisuke,
Torimoto Kazumasa,
Iwasaki Hirotaka,
Iwamoto Takashi,
Morizawa Yosuke,
Hori Shunta,
Miyake Makito,
Tanaka Nobumichi,
Hirayama Akihide,
Nishi Eiichiro,
Fujimoto Kiyohide
Publication year - 2019
Publication title -
luts: lower urinary tract symptoms
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.451
H-Index - 15
eISSN - 1757-5672
pISSN - 1757-5664
DOI - 10.1111/luts.12272
Subject(s) - tadalafil , carbachol , urethra , contraction (grammar) , endocrinology , cgmp specific phosphodiesterase type 5 , urology , medicine , diabetes mellitus , chemistry , streptozotocin , stimulation , erectile dysfunction
Objective This study investigated the effects of tadalafil on the urethra and detrusor in the initial phase of diabetes in rats. Methods Thirty‐six female Sprague‐Dawley rats were assigned to a non‐diabetes (ND), diabetes (D), or tadalafil‐treated diabetes (DT) group (n = 12 per group), with the DT group receiving oral tadalafil (2 mg/kg/d) for 7 days before the experiments. Seven weeks after diabetes induction (by a single intraperitoneal injection of streptozotocin), urethral and intravesical pressure were simultaneously recorded in vivo, whereas responses of detrusor strips to potassium chloride (30 mM), electrical field stimulation (EFS) and carbachol were measured in vitro. Results The intravesical pressure at which the urethra started to relax was significantly lower in the DT than D group (mean [± s.d.] 18.9 ± 2.9 vs 29.1 ± 6.6 cm H 2 O; P < .05). In addition, the reduction in urethral pressure was significantly larger in the DT than D group (−10.9 ± 4.0 vs −4.0 ± 2.9 cm H 2 O; P < .05). Detrusor stimulation revealed that the mean contractile responses to EFS and carbachol were significantly lower in the ND and DT groups than in the D group (120.7 ± 26.5% and 130.8 ± 15.8% vs 200.1 ± 47.9% of the 30 mM KCl‐induced contraction, respectively, in response to 50 Hz EFS [ P < .05]; 211.1 ± 35.4% and 208.4 ± 25.3% vs 425.7 ± 125.0% of the 30 mM KCl‐induced contraction, respectively, in response to 10 −3 M carbachol [ P < .05]). Conclusions Tadalafil restored urethral relaxation function and detrusor responses to EFS and carbachol during the initial phase of diabetes.