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Early cirrhosis and a preserved bone marrow niche favour regenerative response to growth factors in decompensated cirrhosis
Author(s) -
Anand Lovkesh,
Bihari Chhagan,
Kedarisetty Chandan K.,
Rooge Sheetalnath B.,
Kumar Dhananjay,
Shubham Smriti,
Kumar Guresh,
Sahney Amrish,
Sharma Manoj K.,
Maiwall Rakhi,
Kumar Anupam,
Sarin Shiv K.
Publication year - 2019
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/liv.13923
Subject(s) - medicine , ascites , cirrhosis , gastroenterology , bone marrow , stem cell , granulocyte colony stimulating factor , haematopoiesis , erythropoietin , biology , chemotherapy , genetics
Background Exogenous growth factor‐mobilized bone marrow ( BM ) stem cells have shown a differential response in the management of decompensated cirrhosis ( DC ). This study was designed to evaluate potential clinical benefit of adding Erythropoietin ( EPO ) in granulocyte‐colony stimulating factor (G‐ CSF )‐mobilized stem cell therapy, possible mechanisms of regeneration and predictive factors of regenerative response. Methods Sixty consecutive DC patients received either G‐ CSF with EPO (Group A; n = 30) or G‐ CSF and placebo (Group B; n = 30) for 2 months and were carefully followed up for 1 year. Baseline and post‐treatment liver biopsy, BM biopsy and BM aspirate were analysed for fibro‐inflammatory and regenerative response and BM hematopoietic reservoir. Results Addition of EPO to G‐ CSF showed a significant improvement in Child‐Pugh score ( P = 0.03) and MELD score ( P = 0.003) as compared to G‐ CSF alone, with reduction in mortality (16.6% vs 36.7%, P = 0.09). The combination arm also demonstrated a decreased incidence of acute kidney injury ( P < 0.001), encephalopathy ( P = 0.005) and refilling of ascites ( P = 0.03). Compared to monotherapy, it increased CD 163+ macrophages ( P = 0.013), Ki67+ index ( P < 0.001) with decrease in α‐ SMA levels ( P < 0.001) in liver tissue. The response was better with grade 1 and 2 than with grade 3 ascites; Child B cirrhosis and MELD < 16. Non‐responders had lower hematopoietic stem cells ( HSC s) at baseline. On multivariate analysis, the liver disease severity ( MELD < 16) and a relatively preserved BM ( BM ‐ HSC s > 0.4) predicted therapeutic response ( AUROC = 0.82). Conclusions Early DC ( MELD < 16) patients with mild‐moderate ascites and those with a healthy cellular baseline BM respond better to growth factor therapy. Addition of EPO to G‐ CSF provides better regenerative response than G‐ CSF monotherapy.