Premium
Metabolic alterations in a rat model of hepatic ischaemia reperfusion injury: In vivo hyperpolarized 13 C MRS and metabolic imaging
Author(s) -
Moon ChungMan,
Shin SangSoo,
Lim NamYeol,
Kim SeulKee,
Kang YangJoon,
Kim HyoungOok,
Lee SeungJin,
Beak ByungHyun,
Kim YunHeon,
Jeong GwangWoo
Publication year - 2018
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/liv.13695
Subject(s) - in vivo , intravoxel incoherent motion , medicine , alanine , alanine aminotransferase , endocrinology , chemistry , nuclear medicine , effective diffusion coefficient , magnetic resonance imaging , biochemistry , biology , amino acid , microbiology and biotechnology , radiology
Background & Aims Despite a number of studies addressing the pathophysiology of hepatic IRI , a gold standard test for early diagnosis and evaluation of IRI remains elusive. This study investigated the metabolic alterations in a rat model of hepatic IRI using the in vivo hyperpolarized ¹³C MRS and metabolic imaging. Methods Hyperpolarized 13 C MRS with IVIM ‐ DWI was performed on the liver of 7 sham‐operated control rats and 7 rats before and after hepatic IRI . Results The hepatic IRI ‐induced rats showed significantly higher ratios of [1‐ 13 C] alanine/pyruvate, [1‐ 13 C] alanine/ tC , [1‐ 13 C] lactate/pyruvate and [1‐ 13 C] lactate/ tC compared with both sham‐operated controls and rats before IRI , whereas [1‐ 13 C] pyruvate/ tC ratio was decreased in IRI ‐induced rats. In IVIM ‐ DWI study, apparent diffusion coefficient (ADC) , f and D values in rats after hepatic IRI were significantly lower than those of rats before IRI and sham‐operated controls. The levels of [1‐ 13 C] alanine and [1‐ 13 C] lactate were negatively correlated with ADC , f and D values, whereas the level of [1‐ 13 C] pyruvate was positively correlated with these values. Conclusions The levels of [1‐ 13 C] alanine, [1‐ 13 C] lactate and [1‐ 13 C] pyruvate in conjunction with IVIM ‐ DWI will be helpful to evaluate the hepatic IRI as well as these findings can be useful in understanding the biochemical mechanism associated with hepatic damage.