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Peripheral phenotype and gene expression profiles of combined liver–kidney transplant patients
Author(s) -
Dumontet Erwan,
Danger Richard,
Vagefi Parsia A.,
Londoño MariaCarlota,
Pallier Annaïck,
Lozano Juan José,
Giral Magali,
Degauque Nicolas,
Soulillou JeanPaul,
MartínezLlordella Marc,
Lee Herman,
Latournerie Marianne,
Boudjema Karim,
Dulong Joelle,
Tarte Karin,
SanchezFueyo Alberto,
Feng Sandy,
Brouard Sophie,
Conchon Sophie
Publication year - 2016
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/liv.12917
Subject(s) - immunosuppression , phenotype , peripheral blood mononuclear cell , liver transplantation , kidney , gene expression , immune system , kidney transplantation , peripheral , messenger rna , biology , medicine , gene , immunology , transplantation , in vitro , genetics
Background and Aims The beneficial effect of one graft on another has been reported in combined transplantation but the associated mechanisms and biological influence of each graft have not yet been established. Methods In multiple analyses, we explored the PBMC phenotype and signature of 45 immune‐related messenger RNA s and 754 micro RNA s from a total of 235 patients, including combined liver–kidney transplant recipients ( CLK ), patients with a liver (L‐ STA ) or kidney (K‐ STA ) graft only under classical immunosuppression and patients with tolerated liver (L‐ TOL ) or kidney grafts (K‐ TOL ). Results CLK show an intermediary phenotype with a higher percentage of peripheral CD 19 + CD 24 + CD 38 Low memory B cells and Helios + Treg cells, two features associated with tolerance profiles, compared to L‐ STA and K‐ STA ( P < 0.05, P < 0.01). Very few mi RNA were significantly differentially expressed in CLK vs. K‐ STA and even fewer when compared to L‐ STA (35 and 8, P < 0.05). Finally, CLK are predicted to share common mi RNA targets with K‐ TOL and even more with L‐ TOL (344 and 411, P = 0.005). Altogether CLK display an intermediary phenotype and gene profile, which is closer to that of liver transplant patients, with possible similarities with the profiles of tolerant patients. Conclusion These data suggest that CLK patients show the immunological influence of both allografts with liver having a greater influence.