Premium
P311 modulates hepatic stellate cells migration
Author(s) -
Guimarães Eduardo L.,
Stradiot Leslie,
Mannaerts Inge,
Schroyen Ben,
Grunsven Leo A.
Publication year - 2015
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/liv.12691
Subject(s) - hepatic stellate cell , gene knockdown , cell migration , microbiology and biotechnology , chemokine , extracellular matrix , reactive oxygen species , reporter gene , in vivo , chemistry , biology , in vitro , gene expression , biochemistry , immunology , inflammation , endocrinology , gene
Background & Aims Liver fibrosis is induced by the accumulation of extracellular matrix, deposited mainly by activated hepatic stellate cells ( HSC s). One key characteristic of stellate cell activation is the directional migration to the site of injury during the wound‐healing process. P311 is a protein that has been shown to play a role in migration and we aimed to study a possible role for this protein during stellate cell migration. Methods Mouse stellate cells were isolated and cultured in vitro to investigate P311 protein and gene expression during HSC activation by immunocytochemistry and RT‐ qPCR respectively. Expression of P311 during in vivo activation was evaluated in CCl 4 and bile duct ligation‐induced liver fibrosis. Production of reactive oxygen species was determined using the fluorescent probe DCFH‐DA. By siRNA‐mediated knockdown of P311, we investigated a possible effect on proliferation by incorporation of EdU and on migration by Boyden chamber assays. Results P311 gene expression was increased during both in vitro and in vivo activation of HSC s. si RNA ‐mediated knockdown led to a decrease in reactive oxygen production and cell proliferation. Migration induced by different chemokines, such as PDGF ‐bb and MCP ‐1 was inhibited by knockdown of P311. Conclusions P311 is central to reactive oxygen species‐mediated HSC migration induced by different chemokines.