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The micro RNA ‐146a/b attenuates acute small‐for‐size liver graft injury in rats
Author(s) -
Jiang Weiwei,
Ni Qingfeng,
Tan Longwei,
Kong Liangliang,
Lu Yeting,
Xu Xiaoqun,
Kong Lianbao
Publication year - 2015
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/liv.12674
Subject(s) - liver injury , in vivo , medicine , in vitro , pathogenesis , microbiology and biotechnology , pharmacology , immunology , biology , biochemistry
Background & Aims A critical role of the Toll‐like receptor ( TLR )‐4 and its downstream mediators in the pathogenesis of small‐for‐size liver graft injury has been documented. Recently, the micro RNA ‐146 (miR‐146) was identified as a potent negative regulator of the TLR 4 signalling pathway. In this study, the role of miR‐146a and miR‐146b in the attenuation of TLR ‐4 signalling and small‐for‐size liver graft injury was investigated. Methods The expression levels of miR‐146a and miR‐146b during small‐for‐size liver graft injury were studied in vivo . In addition, the effects of miR‐146a and miR‐146b on the expression of IRAK 1 and TRAF 6 in the rat macrophage cell line NR 8383 and rat liver kupffer cells were studied in vitro . The in vivo effect of miR‐146a and miR‐146b on small‐for‐size liver graft injury was studied by the tail vein injection of miR‐146a mimics and miR‐146b mimics. Results The levels of miR‐146a and miR‐146b decreased with a small‐for‐size liver graft. MiR‐146a and miR‐146b inhibited IRAK 1 and TRAF 6 expression by binding to the 3′ UTR of IRAK 1 or TRAF 6, respectively, in the rat macrophage cell line NR 8383. The administration of miR‐146a mimics and miR‐146b mimics prevented liver graft injury in small‐for‐size liver graft injury via the inactivation of IRAK 1 and TRAF 6 in vivo . Conclusions miR‐146a and miR‐146b prevent liver injury in small‐for‐size liver graft injury via the inactivation of IRAK 1 and TRAF 6.