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Platelet increases survival in a model of 90% hepatectomy in rats
Author(s) -
López Mónica L.,
Kieling Carlos O.,
Uribe Cruz Carolina,
Osvaldt Alessandro,
Ochs de Muñoz Gustavo,
Meurer Luise,
Silla Lucia,
Matte Ursula
Publication year - 2014
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/liv.12326
Subject(s) - platelet , liver regeneration , hepatectomy , medicine , serotonin , mitotic index , endocrinology , survival rate , andrology , chemistry , receptor , regeneration (biology) , biology , surgery , mitosis , microbiology and biotechnology , resection
Background & Aims Ninety per cent hepatectomy in rodents is a model for acute liver failure. It has been reported that platelets have a strong effect enhancing liver regeneration, because of the production of several growth factors such as serotonin. The aim of this study was to investigate the role of microencapsulated platelets on 90% hepatectomy in rats. Methods Platelets ( PLT ) were microencapsulated in sodium alginate and implanted in the peritoneum of rats after 90% partial hepatectomy ( PH ). Control group received empty capsules ( EC ). Animals were euthanized at 6, 12, 24, 48 and 72 h post PH ( n = 9–12/group/time) to evaluate liver regeneration rate, mitotic index, liver content, serum and tissue levels of Interleukin 6 ( IL ‐6) and serotonin and its receptor 5‐hydroxytryptamine type 2B ( 5Ht2b ). Survival rate in 10 days was evaluated in a different set of animals ( n = 20/group). Results Platelets group showed the highest survival rate despite the lowest liver regeneration rate at any time point. Mitotic and BrdU index showed no difference between groups. However, the number of hepatocytes was higher and the internuclear distance was shorter for PLT group. Liver dry weight was similar in both groups indicating that water was the main responsible factor for the weight difference. Gene expression of IL ‐6 in the liver was significantly higher in EC group 6 h after PH , whereas 5Ht2b was up‐regulated at 72 h in PLT group. Conclusions Platelets enhance survival of animals with 90% PH , probably by an early protective effect on hepatocytes and the increase in growth factor receptors.