Premium
Preliminary evidence of sustained expression of angiopoietin‐2 during monocyte differentiation in chronic hepatitis C
Author(s) -
RodríguezMuñoz Yolanda,
MartínVílchez Samuel,
LópezRodríguez Rosario,
HernándezBartolomé Ángel,
GarcíaBuey Luisa,
Borque María J.,
MorenoOtero Ricardo,
SanzCameno Paloma
Publication year - 2013
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/liv.12125
Subject(s) - angiopoietin receptor , monocyte , immunology , flow cytometry , angiopoietin , pathogenesis , medicine , antigen , angiogenesis , vascular endothelial growth factor , vegf receptors
Abstract Background Monocytes are essential precursors of antigen‐presenting cells, such as macrophages and dendritic cells, and contribute to the pathogenesis of chronic inflammatory diseases and cancer. Aims As Tie2‐expressing monocytes ( TEM s) are increased in the peripheral blood of patients with chronic hepatitis C ( CHC ), we aimed to examine the expression of Tie2 and angiopoietins (Ang1 and Ang2) during monocyte differentiation and maturation in CHC . Methods The expression of Tie2, CD 11b, CD 80, CD 83, CD 86 and MHC ‐ II was measured by flow cytometry in peripheral blood monocytes and monocytes‐derived cells (Mo‐ DC s) from nine healthy subjects and eight CHC patients whose HCV infection was unresolved after combination therapy. Ang1 and Ang2 levels were measured in cellular supernatants by ELISA . Results Mo‐ DC s from CHC patients expressed differential patterns of maturation markers compared with controls – primarily with regard to CD 80. Tie2 was downregulated during monocyte differentiation in controls and CHC patients, whereas Ang1 expression was constant. However, Ang2 levels fell significantly during the differentiation of control monocytes, in contrast with those from CHC patients in whom Ang2 expression remained stable throughout differentiation. Conclusions Altered expression of the Ang/Tie2 system in monocytes and Mo‐ DC s from CHC patients might account for the inflammatory and angiogenic disorders that are related to CHC . An increased understanding of Ang/Tie2 system regulation might be helpful in designing strategies to prevent CHC progression.