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Insights into collateral susceptibility and collateral resistance in Acinetobacter baumannii during antimicrobial adaptation
Author(s) -
Dawan J.,
Kim J.C.,
Ahn J.
Publication year - 2021
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13488
Subject(s) - acinetobacter baumannii , microbiology and biotechnology , tetracycline , tobramycin , polymyxin b , antibiotics , biology , ciprofloxacin , acinetobacter , efflux , chemistry , bacteria , pseudomonas aeruginosa , genetics , gentamicin
The susceptibility of Acinetobacter baumannii exposed to primary antibiotic can be either increased or decreased when exposed to secondary antibiotic. This study was designed to assess the relative fitness, collateral susceptibility and collateral resistance of polymyxin B‐ (PMB‐) adapted A . baumannii to ciprofloxacin (CIP), meropenem (MER), PMB, tetracycline (TET) and tobramycin (TOB). Strains of wild‐type A . baumannii KACC 12454 (AB KACC ), wild‐type A . baumannii CCARM 12088 (AB CCARM ), PMB‐adapted AB KACC , PMB‐adapted AB CCARM , stabilized AB KACC and stabilized AB CCARM were used in this study. Compared to the wild‐type AB KACC , the MICs of PMB were increased from 2 to 128 μ g ml −1 against PMB‐adapted AB KACC , while MICs of CIP, MER, TET and TOB were decreased from 2 to 1 μ g ml −1 , 16 to 1 μ g ml −1 , 16 to 2 μ g ml −1 and 64 to 16 μ g ml −1 , respectively. The PMB‐adapted AB CCARM was resistant to CIP (32 μ g ml −1 ) and PMB (64 μ g ml −1 ) compared to the wild‐type AB CCARM . The resistance of stabilized AB KACC and AB CCARM to all antibiotics was lost after antibiotic‐free culture in the exception of CIP and TET. The susceptibilities of wild‐type, PMB‐adapted and stabilized AB KACC and AB CCARM to CIP, MER, PMB, TET and TOB were increased in the presence of β‐lactamase and efflux pump inhibitors. The high levels of relative fitness were observed for stabilized AB KACC , PMB‐adapted AB CCARM and stabilized AB CCARM . The stabilized AB KACC and PMB‐adapted AB CCARM were highly heteroresistance to PMB and TET, respectively. The PMB‐adapted AB KACC and AB CCARM showed various antibiotic patterns, known as collateral susceptibility and collateral resistance. The results provide useful information for designing effective antibiotic regimens that can enhance the antibiotic activity against A. baumannii infections.