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Interstrains comparison of the antimicrobial effect and mode of action of a Vietnamese Cinnamomum cassia essential oil from leaves and its principal component against Listeria monocytogenes
Author(s) -
Dumas E.,
Degraeve P.,
Trinh N.T.T.,
Le Thanh M.,
Oulahal N.
Publication year - 2021
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13465
Subject(s) - cinnamomum , cassia , cinnamaldehyde , listeria monocytogenes , chemistry , essential oil , antibacterial activity , minimum inhibitory concentration , antimicrobial , antibacterial agent , membrane permeability , chromatography , microbiology and biotechnology , bacteria , biochemistry , membrane , biology , antibiotics , organic chemistry , medicine , alternative medicine , pathology , traditional chinese medicine , genetics , catalysis
The antibacterial activity of a Cinnamomum cassia essential oil (EO) and of its main component trans ‐cinnamaldehyde (90% w/w) was examined against five Listeria monocytogenes strains. The minimal inhibitory concentrations (MICs) of C . cassia EO against the five L . monocytogenes strains were identical (250 µ g ml −1 ), while the minimal bactericidal concentrations (MBCs) ranged between 800 and 1200 µ g ml −1 . In order to study if this EO and trans ‐cinnamaldehyde altered the five strains at the membrane level, fluorescence anisotropy of 1,6‐diphenyl‐1,3,5‐hexatriene (DPH) was measured in presence of different concentrations (1/2MIC, MIC, 2MIC) of these antibacterial agents. A concentration‐dependent increase of fluorescence anisotropy of DPH in their presence reflecting a rigidification of the membrane was observed for the five strains. This modification of the membrane fluidity was associated with a perturbation of the selective membrane permeability, as a perturbation of the gradient between intracellular and extracellular pH was also observed.