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Bradyrhizobium arachidis mediated enhancement of (oxy)matrine content in the medicinal legume Sophora flavescens
Author(s) -
Wu Z.Y.,
Meng X.F.,
Jiao Y.S.,
Guo B.L.,
Sui X.H.,
Ma S.J.,
Chen W.F.,
Singh R.P.
Publication year - 2021
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13453
Subject(s) - matrine , oxymatrine , rhizobia , sophora flavescens , legume , inoculation , bradyrhizobium , biology , nitrate , bradyrhizobium japonicum , sophora , horticulture , nitrogen fixation , botany , rhizobiaceae , rhizobium , symbiosis , bacteria , medicine , ecology , genetics , alternative medicine , traditional chinese medicine , neuroscience , pathology , pharmacology
Effect of rhizobial inoculation and nitrate application on the content of bioactive compounds in legume plants is an interesting aspect for interactions among microbes, plants and chemical fertilizers, as well as for cultivated practice of legumes. In this study, nitrate (0, 5 and 20 mmol l −1 ) and Bradyrhizobium arachidis strain CCBAU 051107 T were applied, individually or in combination, to the root rhizosphere of the medicinal legume Sophora flavescens Aiton (SFA). Then the plant growth, nodulation and active ingredients including (oxy)matrine of SFA were determined and compared. Rhizobial inoculation alone significantly increased the numbers and fresh weight of root nodules. Nodulation was significantly inhibited due to nitrate (5 and 20 mmol l −1 ). Only oxymatrine was detected in the control plants without rhizobial inoculation and nitrate supplement, while both oxymatrine and matrine were synthesized in plants treated with inoculation of B. arachidis or supplied with nitrate. The content of oxymatrine was the highest in plants inoculated solely with rhizobia and was not significantly altered by additional application of nitrate. Combinations of B. arachidis inoculation and different concentrations of nitrate did not significantly change the concentrations of (oxy)matrine in the plant. In conclusion, sole rhizobial inoculation was the best approach to increase the contents of key active ingredients oxymatrine and matrine in the medicinal legume SFA.