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Characterization and application of rhamnolipid from Pseudomonas plecoglossicida BP03
Author(s) -
Sabarinathan D.,
Vanaraj S.,
Sathiskumar S.,
Poorna Chandrika S.,
Sivarasan G.,
Arumugam S.S.,
Preethi K.,
Li H.,
Chen Q.
Publication year - 2021
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13403
Subject(s) - rhamnolipid , microbiology and biotechnology , bacillus subtilis , staphylococcus aureus , antimicrobial , cytotoxicity , biology , bacteria , pseudomonas aeruginosa , docking (animal) , food science , chemistry , biochemistry , in vitro , genetics , medicine , nursing
The production of rhamnolipid (glycolipid) biosurfactant was achieved under optimized conditions from newly isolated bacteria ( Pseudomonas plecoglossicida BP03 ) from rice mill effluent. The isolated biosurfactant was structurally characterized using FTIR and NMR spectroscopic studies. The obtained biosurfactant (1·39 g l −1 ) showed a variety of applications including larvicidal and pupicidal activity against malarial vector ( Anopheles sunadicus ). It also exhibited antimicrobial activity against human pathogens, and possessed potent anti‐biofilm activity against Staphylococcus aureus , Bacillus subtilis and Aeromonas hydrophila . The obtained biosurfactant showed a dose‐dependent inhibition of exopolymeric substance (EPS) and growth curve in S. aureus . Furthermore, the cytotoxicity assays revealed that the biosurfactant exhibit a cytotoxic potency against the human fibroblastic sarcoma cells Ht‐1080. An in silco analysis was also performed using Schrodinger maestro 9.3 against surface protein (SasG) of S. aureus , and the resultant analysis revealed an interactive docking score of −3·4 kcal mol −1 . The obtained result indicates that the synthesized economically viable biosurfactant ensures excellent applications towards various fields.