Premium
Disruption of glpF gene encoding the glycerol facilitator improves 1,3‐propanediol production from glucose via glycerol in Escherichia coli
Author(s) -
Sato R.,
Tanaka T.,
Ohara H.,
Aso Y.
Publication year - 2021
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13391
Subject(s) - glycerol , escherichia coli , biochemistry , biology , chemistry , food science , gene
Engineered Escherichia coli has recently been applied to produce 1,3‐propanediol (1,3‐PDO) from glucose. A metabolic intermediate in the production pathway, glycerol, is partially secreted into the extracellular of E. coli through a glycerol facilitator encoded by glpF , and this secretion consequently decreases 1,3‐PDO production. Therefore, we aimed to determine whether disrupting the glpF gene would improve 1,3‐PDO production in E. coli . The intracellular glycerol concentration in a glpF ‐disruptant was 7·5 times higher than in a non‐disruptant. The glpF ‐disrupted and non‐disrupted E. coli strains produced 0·26 and 0·09 g l −1 of 1,3‐PDO, respectively, from 1% glucose after 72 h of cultivation. The specific growth rate (μ) and the 1,3‐PDO yield from glucose ( Y P/S ) in the disruptant were higher than those in the non‐disruptant ( ΔglpF , μ = 0·08 ± 0·00 h −1 , Y P/S = 0·06 mol mol‐glucose −1 ; BW25113, μ = 0·06 ± 0·00 h −1 , Y P/S = 0·02 mol mol‐glucose −1 ). Disruption of the glpF gene decreased the production of the by‐product, acetic acid. These results indicated that disruption of glpF increased the intracellular concentration of glycerol and consequently increased 1,3‐PDO production in E. coli .