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Improving probiotic spore yield using rice straw hydrolysate
Author(s) -
Yin L.,
Chen M.X.,
Zeng T.H.,
Liu X.M.,
Zhu F.,
Huang R.Q.
Publication year - 2021
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13387
Subject(s) - bacillus coagulans , hydrolysate , spore , response surface methodology , straw , food science , yeast extract , chemistry , fermentation , biology , botany , biochemistry , agronomy , chromatography , hydrolysis
Spore‐forming Bacillus sp. has been extensively studied for their probiotic properties. In this study, an acid‐treated rice straw hydrolysate was used as carbon source to produce the spores of Bacillus coagulans . The results showed that this hydrolysate significantly improved the spore yield compared with other carbon sources such as glucose. Three significant medium components including rice straw hydrolysate, MnSO 4 and yeast extract were screened by Plackett–Burman design. These significant variables were further optimized by response surface methodology (RSM). The optimal values of the medium components were rice straw hydolysate of 27% (v/v), MnSO 4 of 0·78 g l −1 and yeast extract of 1·2 g l −1 . The optimized medium and RSM model for spore production were validated in a 5 l bioreactor. Overall, this sporulation medium containing acid‐treated rice straw hydrolysate has a potential to be used in the production of B. coagulans spores.