Premium
Genotyping and antibiotic resistance patterns of Campylobacter fetus subsp. venerealis from cattle farms in India
Author(s) -
Ishtifaq A.,
Qureshi S.,
Farooq S.,
Kashoo Z.A.,
Malik Md Z.,
Alam M.R.,
Wani S.A.,
Bhat M.A.,
Hussain M.I.,
Dar R.A.,
Shah S.M.
Publication year - 2020
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13378
Subject(s) - biology , semen , pulsed field gel electrophoresis , genotyping , veterinary medicine , microbiology and biotechnology , preputial gland , campylobacter , campylobacter fetus , multilocus sequence typing , genotype , bacteria , genetics , medicine , gene , endocrinology
Bovine genital campylobacteriosis caused by Campylobacter fetus subsp. venerealis (Cfv) is of considerable economic importance to the cattle industry worldwide. Cfv causes syndrome of temporary infertility in female cattle, early embryonic mortality, aberrant oestrus cycles, delayed conception, abortions and poor calving rates. In the present study, a total of 200 samples obtained from vaginal swabs, cervicovaginal mucous (CVM), preputial washes and semen straws were investigated that were obtained from organized cattle farm of MLRI, Manasbal and unorganized sectors. Out of a total of 200 samples, 49 (47·57%) vaginal swabs, 1 (3·33%) preputial wash and 8 (25%) carried out CVM samples were positive for Cfv, whereas none of the semen straws were positive for Cfv. A total of eleven isolates of Cfv were recovered. PFGE (Pulse field gel electrophoresis) analysis revealed four different pulsotypes (I–IV) circulating in the screened farms. A common pulsotype circulating among farms could not be established. Insertion element ( ISCfe 1), a 233 bp amplicon of Cfv, was sequenced and the sequence was deposited in GenBank (accession no: MK475662).