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Manila clam ( Ruditapes philippinarum ) marketed in Korea as a source of vibrios harbouring virulence and β‐lactam resistance genes
Author(s) -
Dahanayake P.S.,
Hossain S.,
Wickramanayake M.V.K.S.,
Wimalasena S.H.M.P.,
Heo G.J.
Publication year - 2020
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13229
Subject(s) - biology , microbiology and biotechnology , virulence , vibrio , vibrio alginolyticus , coagulase , gene , bacteria , staphylococcus , staphylococcus aureus , genetics
Abstract The present study assessed the occurrence, virulence determinants and antimicrobial susceptibility patterns of Vibrio spp. isolated from live Manila clam ( Ruditapes philippinarum ). A total of 31 Vibrio spp. including 27 V . diabolicus , two V. fluvialis , one V. alginolyticus and one V. antiquarius were isolated and identified. Phenotypic detection of DNase, lipase, phospholipase, amylase and caseinase activities was 100%; and 87% gelatinase, 45% slime production and 6% haemolysin activities were also observed. The prevalence of toxin‐related virulence genes for collagenase (94%), toxR (100%), tlh (68%), and VPI (71%) was detected by PCR . Additionally, two V. fluvialis isolates carried F‐toxR and hupo genes. Moreover, 61% of the isolates showed multiple antimicrobial resistance indices >0·2. The resistance rates of ampicillin, piperacillin, colistin sulfate, rifampicin, and cephalothin were 100, 81, 71, 77 and 68% respectively. The prevalence of blaCTX‐M (87%), blaTEM (55%) and Int1 (90%) genes was observed, whereas blaSHV , strA‐strB , tetA , tetB , and aadA2 gene cassette were reported in varying combinations. However, armA , aac(3)‐IIa and quinolone resistance genes ( qnrA , qnrB , qnrS ) were not amplified. Thus, the virulence along with extended‐spectrum β‐lactamases (ESBLs) and other antimicrobial resistance genes in multidrug‐resistant Manila clam‐borne vibrios may pose a public health threat for consumers. Significance and Impact of the Study In Korea, eating raw seafood is considered a great delicacy. This has negatively affected the public by increasing health issues over the years due to the ingestion of vibrios. For the first time, we could identify Vibrio diabolicus and Vibrio antiquarius in marketed Manila clams in Korea. The prevalent Vibrio diabolicus isolates demonstrated the Vibrio parahaemolyticus and Vibrio cholerae homologous virulence genes ( toxR , tlh , and VPI ) . Additionally, the abundance of extended‐spectrum β‐lactamases (ESBLs) and integron ( IntI1 ) harboured by Manila clam‐borne vibrios elucidate the potential health risk for consumers and may complicate health treatments in the case of infection.