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Extended‐spectrum beta‐lactamase‐producing Escherichia coli harbouring sul and mcr ‐ 1 genes isolates from fish gut contents in the Mekong Delta, Vietnam
Author(s) -
Hoa T.T.T.,
Nakayama T.,
Huyen H.M.,
Harada K.,
Hinenoya A.,
Phuong N.T.,
Yamamoto Y.
Publication year - 2020
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13222
Subject(s) - escherichia coli , biology , beta lactamase , microbiology and biotechnology , mcr 1 , colistin , gene , antibiotic resistance , antimicrobial , antibiotics , phylogenetic tree , enterobacteriaceae , genetics
This study investigated the existence of sulfonamides and colistin resistance genes among extended‐spectrum beta‐lactamase (ESBL)‐producing Escherichia coli recovered from fish gut in Vietnam and evaluated the susceptibility patterns of the ESBL‐producing E. coli to relevant antimicrobials. A total of 88 ESBL‐producing E. coli isolates were analysed for the presence of the ESBLs, sul ( 1 , 2 , 3 ) and mcr ( 1 – 3 ) genes by PCR. Antimicrobial resistance phenotypes of isolates were determined by disc diffusion. Results showed that: (i) A high prevalence of 94·3% of sulfonamide resistance was observed in 88 isolates. Moreover, the existence of 2·3% of ESBL‐producing E. coli harbouring mcr ‐ 1 gene were detected; (ii) The phylogenetic types A and B1 were most frequent, and the bla CTX‐M group1 and bla TEM genes encoding ESBL were detected in 47·7% of the isolates; (iii) ESBL‐producing E. coli harbouring mcr ‐ 1 gene exhibited resistance to 11 antibiotics. The existence of mcr ‐ 1 and sul1,2,3 genes and the extremely high level of multiple drug resistance in all ESBL‐producing E. coli isolates obtained from sampled fish in Vietnam is a major concern. Therefore, it is imperative to monitor ESBL‐producing E. coli in the river waters of Vietnam.