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Loop‐mediated isothermal amplification for paratyphoid fever – a proof‐of‐principle analysis
Author(s) -
Rojak S.,
Wiemer D.F.,
Wille A.,
Loderstädt U.,
Wassill L.,
Hinz R.,
Hagen R.M.,
Frickmann H.
Publication year - 2019
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13130
Subject(s) - loop mediated isothermal amplification , paratyphoid fever , microbiology and biotechnology , enteric fever , bacteria , biology , virology , typhoid fever , genetics , dna
In‐house loop‐mediated isothermal amplification ( LAMP ) procedures for the detection of paratyphoid fever‐associated bacteria on serovar level were evaluated. Therefore, LAMP primers for Salmonella genus, for two LAMP schemes for S . Paratyphi A, for S . Paratyphi B and for S . Paratyphi C were tested with DNA from culture isolates from strain collections and spiked blood cultures against published PCR protocols targeting the same micro‐organisms. Sensitivity and specificity for DNA from culture isolates verified by LAMP ranged from 80·0 to 100·0% and 96·1 to 100·0% vs 65 to 100% and 98·7 to 100% for the PCR approaches. For the spiked blood culture materials, sensitivity and specificity for LAMP ranged from 87·5 to 100·0% and 96·7 to 100·0% vs from 60 to 100% and 98·2 to 100% for PCR . In conclusion, LAMP for paratyphoid fever shows comparable performance characteristics as PCR . Due to its easy application, the procedure is well suited for surveillance purposes in resource‐limited settings. Significance and Impact of the Study The use of easy‐to‐apply, point‐of‐care‐testing‐like loop‐mediated isothermal amplification ( LAMP ) for the diagnosis of paratyphoid fever is evaluated. This approach can contribute to low‐threshold availability of surveillance options for resource limited settings. Easy‐to‐teach and easy‐to‐apply LAMP schemes with similar performance characteristics as PCR are provided. The described test evaluation is of particular use for surveillance and public health experts.