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Effect of photopolymerized glaze application on bacterial adhesion on ocular acrylic resin surfaces submitted to accelerated ageing
Author(s) -
Nagay B.E.,
Goiato M.C.,
da Silva E.V.F.,
Andreotti A.M.,
Bitencourt S.B.,
Duque C.,
Santos P.H.,
Santos D.M.
Publication year - 2019
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.13094
Subject(s) - glaze , staphylococcus epidermidis , adhesion , ageing , biofilm , surface roughness , surface finish , materials science , bacterial growth , staphylococcus aureus , chemistry , microbiology and biotechnology , composite material , bacteria , medicine , biology , ceramic , genetics
Conditions of the acrylic resin ( AR ) surface, such as roughness, can promote a favourable environment for the adhesion of micro‐organisms, even on the surface of ocular prostheses. This study evaluated the influence of photopolymerized glaze application on the roughness of AR s and adhesion of Staphylococcus aureus and Staphylococcus epidermidis on ocular AR surfaces submitted to accelerated ageing. Two hundred and eighty‐eight samples of white colour (N1) and colourless AR s were distributed in eight groups ( n = 9), based on surface treatments (glaze or AR s submitted to only a final polishing), accelerated ageing (before and after) and periods of microbial growth (24‐ and 48‐h). The roughness average (Ra) and total height of roughness profile (Rt) values were greater for the groups with glaze and increased for all groups after ageing. The microbial adhesion among the groups with and without glaze did not present a statistically significant difference. The ageing did not statistically affect the adhesion of Staph. epidermidis , but affected the adhesion of Staph. aureus , which presented an increase after 24 h of growth on only N1 AR with glaze. These results demonstrate that the glaze did not contribute to adhesion of Staph. aureus and Staph. epidermidis , which are responsible for most ocular prosthetic infections. Significance and Impact of the Study Some recent evidence suggested that the surface finish of ocular prostheses influences the accumulation of deposits that can affect the interaction with pathogenic bacteria, increasing the probability of infections. In addition, surface deterioration over time can increase the roughness and, consequently, biofilm formation. Thus, a better understanding of the influence of surface finish on bacterial adhesion becomes extremely important. In this study, we tested a glaze for surface polishing compared to mechanical polishing, before and after ageing. The results suggest that the glaze did not contribute to microbial adhesion and might be useful in preventing possible prosthetic infections.