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Comparative genome analysis and characterization of the Salmonella Typhimurium strain CCRJ _26 isolated from swine carcasses using whole‐genome sequencing approach
Author(s) -
Panzenhagen P.H.N.,
Cabral C.C.,
Suffys P.N.,
Franco R.M.,
Rodrigues D.P.,
ConteJunior C.A.
Publication year - 2018
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12859
Subject(s) - virulence , prophage , biology , pathogenicity island , genome , plasmid , whole genome sequencing , salmonella , bacterial genome size , genetics , genomic island , microbiology and biotechnology , mobile genetic elements , gene , bacteria , escherichia coli , bacteriophage
Salmonella pathogenicity relies on virulence factors many of which are clustered within the Salmonella pathogenicity islands. Salmonella also harbours mobile genetic elements such as virulence plasmids, prophage‐like elements and antimicrobial resistance genes which can contribute to increase its pathogenicity. Here, we have genetically characterized a selected S . Typhimurium strain ( CCRJ _26) from our previous study with Multiple Drugs Resistant profile and high‐frequency PFGE clonal profile which apparently persists in the pork production centre of Rio de Janeiro State, Brazil. By whole‐genome sequencing, we described the strain's genome virulent content and characterized the repertoire of bacterial plasmids, antibiotic resistance genes and prophage‐like elements. Here, we have shown evidence that strain CCRJ _26 genome possible represent a virulence‐associated phenotype which may be potentially virulent in human infection. Significance and Impact of the Study Whole‐genome sequencing technologies are still costly and remain underexplored for applied microbiology in Brazil. Hence, this genomic description of S . Typhimurium strain CCRJ _26 will provide help in future molecular epidemiological studies. The analysis described here reveals a quick and useful pipeline for bacterial virulence characterization using whole‐genome sequencing approach.