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Improvement in solvent tolerance by exogenous glycerol in Pseudomonas sp. BCNU 106
Author(s) -
Choi H.J.,
Lim B.R.,
Park Y.J.,
Joo W.H.
Publication year - 2017
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12754
Subject(s) - glycerol , toluene , biodegradation , solvent , pseudomonas , bacteria , biotransformation , chemistry , food science , chromatography , microbiology and biotechnology , organic chemistry , biology , genetics , enzyme
Solvent hypertolerant Pseudomonas sp. BCNU 106 still has some underlying growth limitation in solvents. Therefore, efficient mass cultivation methods are needed to pursue its applications in biotechnology. Pseudomonas sp. BCNU 106 was cultured in a medium supplemented with 0·05 mol l −1 glycerol and cell survival was monitored during its cultivation in the presence of 1% (v/v) toluene. Exogenously supplemented glycerol provided more protection against damage caused by toluene stress and conferred higher solvent tolerance of Pseudomonas sp. BCNU 106 to toluene compared to control Pseudomonas sp. BCNU 106 without the supplementation of glycerol. This low‐cost mass cultivation method can be used to efficiently apply solvent‐tolerant bacteria in biotransformation and biodegradation. Significance and Impact of the Study Protection against toluene and improvement in bacterial cell growth by supplementation of glycerol in the presence of toluene are demonstrated in this study. This result can be used to solve growth‐related hindrances of solvent‐tolerant bacteria and establish their low‐cost mass cultivation, thereby broadening their industrial and environmental applications.