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A medicinal herb Cassia alata attenuates quorum sensing in Chromobacterium violaceum and Pseudomonas aeruginosa
Author(s) -
Rekha P.D.,
Vasavi H.S.,
Vipin C.,
Saptami K.,
Arun A.B.
Publication year - 2017
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12710
Subject(s) - chromobacterium violaceum , quorum sensing , pseudomonas aeruginosa , microbiology and biotechnology , virulence , biofilm , cassia , biology , bacteria , pyocyanin , biochemistry , gene , medicine , genetics , alternative medicine , traditional chinese medicine , pathology
Quorum sensing ( QS ) has been shown to play a crucial role in the pathogenesis in many bacteria, and attenuation of QS is one of the targets of antimicrobial therapy with particular interest in combating drug resistance. This study reports the QS inhibitory activity of metabolites from Cassia alata L. ( Ca. alata ), an important medicinal herb widely used in the treatment of microbial infections. For investigating the QS inhibition ( QSI ), the potential of Ca. alata L., initially, metabolites of the leaves extracted using ethanol was tested against biosensor strain Chromobacterium violaceum CV 026 and C. violaceum wild‐type strains. Furthermore, a purified fraction rich in flavonoids (F‐ AF ) was used for establishing QSI activity by studying the inhibition of violacein production in C. violaceum , and QS controlled virulence and biofilm formation in Pseudomonas aeruginosa PAO 1. The study results showed 50% inhibition of violacein production in C. violaceum at 0·05 mg ml −1 concentration of F‐ AF . In P. aeruginosa PAO 1, it inhibited the tested virulence factors and biofilm formation significantly. The F‐ AF contained major flavonoids namely, quercetin, quercetrin and kaempferol displaying QSI activity individually against the test organisms. Significance and Impact of the Study Present study demonstrates the quorum sensing inhibitory activity of metabolites from Cassia alata , an important medicinal herb which is commonly used worldwide in the treatment of infections caused by microorganisms. An extract prepared from the leaves of the plant showed activity against quorum sensing in Chromobacterium violaceum and was also effective against attenuating the quorum sensing controlled virulence factors in Pseudomonas aeruginosa . Activity is attributed to the rich flavonoid composition of the plant. Results of the present investigation throw an insight into the possibility of developing drug formulations using the isolated compounds against infections caused by quorum sensing‐mediated pathogenicity of bacteria.