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Enhancing nosiheptide production in Streptomyces actuosus by heterologous expression of haemoprotein from Sinorhizobium meliloti
Author(s) -
Mo Q.,
Zhang H.,
Liu Q.,
Tang X.,
Zhao L.,
Zan X.,
Song Y.
Publication year - 2016
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12575
Subject(s) - strain (injury) , heterologous , biology , mutant , recombinant dna , sinorhizobium meliloti , heterologous expression , fermentation , biochemistry , hemeprotein , gene , heme , enzyme , anatomy
Oxygen deficiency is a critical limiting factor for nosiheptide production in Streptomyces actuosus during fermentation. To alleviate oxygen limitation and enhance the yield of nosiheptide, haemoprotein from Sinorhizobium meliloti (SmHb) was overexpressed in S. actuosus with overexpression of haemoglobin from Vitreoscilla ( VH b) as a positive control. The expression of SmHb and VH b in S. actuosus was confirmed by SDS ‐ PAGE and CO ‐difference spectra analysis. The results showed that S. actuosus recombinant strain with SmHb expression had higher nosiheptide production (increased by 151%) than the wild strain ( WT ) under the low aeration conditions, which was similar with S. actuosus mutant strain with VH b expression. Furthermore, two copies of SmfHb gene were integrated in S. actuosus , which further increased the nosiheptide production by approx. 1·9‐fold compared with original strain, and final nosiheptide yield was up to 2352 μ g ml −1 . These results suggested that engineering of SmHb expression could be used as an efficient method for constructing a high nosiheptide‐accumulating strain. Significance and Impact of the Study The significant improvement of nosiheptide production was found in recombinant strain with overexpressed sm gene. And further improvement was obtained in the two copies of sm overexpressing strain. These results suggested that engineering of SmHb expression could be used as an efficient method for constructing a high nosiheptide‐accumulating strain.