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Enhancement of solvent tolerance in Pseudomonas sp. BCNU 106 with trehalose
Author(s) -
Lim B.R.,
Choi H.J.,
Kwon G.S.,
Joo W.H.
Publication year - 2015
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12504
Subject(s) - trehalose , biodegradation , biotransformation , solvent , toluene , bacteria , pseudomonas , chemistry , bacterial growth , biochemistry , biology , microbiology and biotechnology , food science , organic chemistry , enzyme , genetics
As the solvent hyper‐resistant Pseudomonas sp. BCNU 106 experiences limited growth with solvents, a strategy is therefore needed to allow better growth to broaden its performance in biotechnological applications. Pseudomonas sp. BCNU 106 was cultivated in a medium supplemented with 0·05 mol l −1 trehalose, and the cell survival was observed during subsequent growth with 1% (v/v) toluene. Exogenously added trehalose was transported into the cells and conferred protection against toluene stress. BCNU 106 grown in the presence of exogenous trehalose showed higher solvent tolerance, it can thus have more potential for biotransformation and biodegradation. Significance and Impact of the Study This study shows that exogenously supplemented trehalose confers protection against toluene stress and enhances the bacterial cell growth in the presence of toluene. This is of importance to the mass cultivation of solvent‐tolerant bacteria, where some of the growth‐related limitations of solvent‐tolerant bacteria can be overcome, and their performance in biotechnological applications for biotransformation and biodegradation broadened.